TABLE 1.

Reactivity of mouse MAbs against DC-SIGN and L-SIGN

Antibody clonea% Positive cells (mean fluorescence)bReactivitycIsotype
NIH 3T3/DC-SIGNNIH 3T3/L-SIGN
507(D)99.2 (512)0.2 (30)DC-SIGN, mac-DC-SIGNIgG2b
516(D)98.8 (169)0.05 (21)DC-SIGN, mac-DC-SIGNIgG2a
531(D)97.8 (254)0.0 (NA)DC-SIGNIgG1
518(X)98.5 (66)95.4 (92)DC-SIGN, L-SIGNIgG2a
526(X)98.8 (170)98.6 (175)DC-SIGN, L-SIGN, mac-DC-SIGNIgG2a
612(X)98.8 (182)99.1 (309)DC-SIGN, L-SIGNIgG2a
604(L)1.3 (40)99.5 (650)L-SIGNIgG2b
IgG10.4 (27)0.0 (NA)NA
IgG2a0.2 (27)0.0 (NA)NA
IgG2b0.4 (29)0.03 (22)NA
  • a The MAb clones share a three-digit prefix of 120XXX; the last three digits were used to distinguish the clones. Mouse IgG isotype controls were also examined.

  • b Staining of parental NIH 3T3 cells was uniformly negative (data not shown).

  • c Mac, Rhesus macaque (33); NA, not applicable.