TABLE 4.

oriS is required in both amplicon plasmids for efficient IM-HR

Amplicon plasmidsNo. of blue coloniesa% IM-HR
AmprKanr
pW7 + pW2-lacZ32474143.7
pW7-del + pW2-lacZ27000.3
pW7 + pW2-lacZ-del81127.1
pW7-del + pW2-lacZ-del02000
  • a Values are averages for two repeated experiments.