TABLE 1.

IC50 of a panel of monoclonal antibodies against HIV-1 89.6 pseudotyped with WT or mutant Env

EnvIC50 (μg/ml)a
CD4bV3 (447-52D)CD4iMannan (2G12)gp41 MPER
IgG1b12CD4-IgG17b without sCD417b with sCD4b2F54E10
WT0.460.943.84>20.00>20.002.374.496.62
N60.1380.521.18>20.00>20.002.643.68.81
N70.01630.0650.311.010.630.752.262.22
NV30.1430.52>4.84>20.00>20.004.834.483.3
N67V30.00690.0470.0370.240.591.615.21.97
  • a Input virus was normalized by their infectivity at 100 TCID50 in TZM-bl cells. The IC50 is defined as the concentration at which 50% reduction of the viral infectivity was achieved. These results represent the average of two or more experiments. The column subheadings indicate the respective epitopes and monoclonal antibodies.

  • b A subneutralizing concentration of sCD4 was used for these assays. Since the WT and mutant viruses exhibit different sensitivities to sCD4, the concentration of sCD4 used was determined empirically for each indicator virus: WT, 0.15 μg/ml; N6, 0.02 μg/ml; N7, 0.002 μg/ml; NV3, 0.02 μg/ml; and N67V3, 0.001 μg/ml.