TABLE 1.

Select statistically significant mRNA levels that decrease ≥1.5-fold after treatment with Bay11-7082 or LY294002 prior to HCMV infectiona

Category and full gene product nameGene product designationProbe set IDFold change in mRNA level by treatment with:
Bay11-7082bLY294002b
Antiapoptotic factors
    Baculoviral IAP repeat-containing 2BIRC236578_at−1.8
    BCL2-related protein A1BCL2A12002_s_at−1.6
    Beclin 1 (coiled-coil, myosin-like BCL2 interacting protein)BECN139378_at−1.9
    CASP8 and FADD-like apoptosis regulatorCFLAR1867_at−2.8
    Chemokine (C-C motif) ligand 2CCL2874_at−3.5−55.2
    Heat shock 70-kDa protein 9B (mortalin 2)HSPA9B41510_s_at−1.9
    Immediate early response 3IER31237_at−2.0
    Interleukin 10IL-101548_s_at−8.4
    Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 (p105)NFKB11377_at−2.8
    Secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1)SPP12092_s_at−4.7−18.5
    Serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 9SERPINB92092_s_at−4.8
    Suppressor of cytokine signaling 3SOCS334438_at−2.5
    Tumor necrosis factor (tumor necrosis factor superfamily, member 2)TNF40968_at−4.1
    Tumor necrosis factor, alpha-induced protein 3TNFAIP31852_at−4.0
    Tumor necrosis factor, alpha-induced protein 8TNFAIP8595_at−4.4
    Tumor rejection antigen (gp96) 1TRA133243_at−1.67
Cell adhesion
    ADAM-like, decysin 1ADAMDEC134974_at−3.1−9.3
    Angiogenic factor VG5QVG5Q35067_at−1.7
    Cadherin 1, type 1, E-cadherin (epithelial)CDH1977_s_at−20.7
    Catenin (cadherin-associated protein), delta 1CTNND140444_s_at−2.0
    CD44 antigen (homing function and Indian blood group system)CD4431472_s_at−1.5
    CD47 antigen (Rh-related antigen, integrin-associated signal transducer)CD4737890_at−2.6
    CD9 antigen (p24)CD939389_at−2.5
    Chemokine (C-C motif) ligand 2CCL234375_at−3.5−55.2
    Chemokine (C-C motif) ligand 4CCL436674_at−7.1
    Complement component 1, q subcomponent, receptor 1C1QR135036_at−4.6
    Endoglin (Osler-Rendu-Weber syndrome 1)ENG32562_at−4.4
    Heat shock 60-kDa protein 1 (chaperonin)HSPD137720_at−2.0
    Integrin, alpha 1ITGA137484_at−2.3−9.6
    Integrin, alpha 6ITGA641266_at−2.52.5
    Integrin, beta 8ITGB8889_at−7.9−5.5
    Intercellular adhesion molecule 1 (CD54), human rhinovirus receptorICAM132640_at−4.7
    Laminin, beta 3LAMB336929_at−2.6−3.7
    Ninjurin 1NINJ141475_at−2.0−2.1
    Oxidized low-density lipoprotein (lectin-like) receptor 1OLR137233_at−1.6
    Pleckstrin homology domain containing, family C (with FERM domain) member 1PLEKHC136577_at−2.7
    Polycystic kidney disease 2 (autosomal dominant)PKD238120_at−2.4
    RAB13, member RAS oncogene familyRAB1340210_at−4.9−2.0
    Scavenger receptor class F, member 1SCARF140034_r_at−3.6
    Secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1)SPP12092_s_at−4.7−18.5
    Thrombospondin 1THBS1115_at−8.9−3.1
    Tumor necrosis factor (TNF superfamily, member 2)TNF1852_at−4.1
    Tumor necrosis factor, alpha-induced protein 6TNFAIP61372_at−14.3
Cell motility
    CD9 antigen (p24)CD939389_at−2.5
    Chemokine (C-C motif) ligand 3CCL336103_at−3.9
    Chemokine (C-C motif) ligand 4CCL436674_at−7.1
    Complement component 3a receptor 1C3AR132068_at−4.2
    Interleukin 10IL-101548_s_at−8.4
    Lymphocyte-specific protein 1LSP136493_at−2.4
    Myristoylated alanine-rich protein kinase C substrateMARCKS32434_at−1.8
    Phosphatase and tensin homolog (mutated in multiple advanced cancers 1)PTEN39552_at−2.0
    Prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase)PTGS21069_at−5.7
    Rho guanine nucleotide exchange factor (GEF) 11ARHGEF1139281_at−2.1
    Secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1)SPP12092_s_at−4.7−18.5
    Thrombospondin 1THBS1115_at−8.9−3.1
Cytokine and chemokine activity
    ATPase, H+ transporting, lysosomal accessory protein 1ATP6AP135770_at−2−1.5
    Chemokine (C-C motif) ligand 18 (pulmonary and activation-regulated)CCL1832128_at−3.6
    Chemokine (C-C motif) ligand 2CCL2874_at−3.5−55.2
    Chemokine (C-C motif) ligand 20CCL2040385_at−11.6
    Chemokine (C-C motif) ligand 22CCL2234041_at−20.2
    Chemokine (C-C motif) ligand 23CCL2336444_s_at−4.6
    Chemokine (C-C motif) ligand 3CCL336103_at−3.9
    Chemokine (C-C motif) ligand 4CCL436674_at−7.1
    Chemokine (C-C motif) ligand 7CCL739802_at−23.5
    Chemokine (C-C motif) ligand 8CCL837823_at−1.9
    Colony-stimulating factor 2 (granulocyte-macrophage)CSF21400_at−2.9
    Colony-stimulating factor 3 (granulocyte)CSF31334_s_at−39.0
    GranulinGRN41198_at−1.8
    Inhibin, beta A (activin A, activin AB alpha polypeptide)INHBA40357_at−6.9
    Interferon, alpha 1IFNA11666_at−5.7
    Interferon, alpha 10IFNA101022_f_at−3.0
    Interferon, alpha 13IFNA131540_f_at−5.9
    Interferon, alpha 2IFNA21791_s_at−3.4
    Interferon, alpha 4IFNA41580_f_at−10.7
    Interleukin 1 receptor antagonistIL1RN37603_at−5.2
    Interleukin 10IL-101548_s_at−8.5
    Interleukin 6 (interferon, beta 2)IL-638299_at−13.6
    Lymphotoxin beta (TNF superfamily, member 3)LTB40729_s_at−1.8
    Macrophage migration inhibitory factor (glycosylation-inhibiting factor)MIF895_at−1.7
    Oncostatin MOSM1579_at−11.6
    Secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1)SPP12092_s_at−4.7−18.5
    Tumor necrosis factor (ligand) superfamily, member 10TNFSF101715_at−5.6
    Tumor necrosis factor (ligand) superfamily, member 8TNFSF833012_at−3.4
    Tumor necrosis factor (TNF superfamily, member 2)TNF1852_at−4.1
Differentiation
    AgrinAGRN33454_at−1.9
    Angiogenic factor VG5QVG5Q35067_at−1.7
    Aryl-hydrocarbon receptor nuclear translocator 2ARNT235352_at−24.3
    CD80 antigen (CD28 antigen ligand 1, B7-1 antigen)CD8035015_at−4.8
    CD9 antigen (p24)CD939389_at−2.5
    Colony-stimulating factor 3 (granulocyte)CSF31334_s_at−39.0
    Fibroblast growth factor 2 (basic)FGF21828_s_at−7.9
    fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor)FLT11545_g_at−6.4
    Histone deacetylase 9HDAC937483_at−7.8−18.7
    Inhibin, beta A (activin A, activin AB alpha polypeptide)INHBA37483_at−6.9
    Interferon, gamma-inducible protein 16IFI1640357_at−2.3
    Interleukin 10IL-101456_s_at−8.4
    Phosphatidylserine receptorPTDSR1548_s_at−2.0
    ras homolog gene family, member HRHOH35851_g_at−2.0
    Secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1)SPP137416_at−4.7−18.5
    SH2 domain protein 2ASH2D2A34432_at−2.7
    Tumor necrosis factor, alpha-induced protein 2TNFAIP238631_at−3.1
Extracellular matrix
    AgrinAGRN33454_at−1.9
    Laminin, beta 3LAMB336929_at−2.6−3.7
    Matrix metalloproteinase 14 (membrane-inserted)MMP14160020_at−2.7
    Matrix metalloproteinase 7 (matrilysin, uterine)MMP7668_s_at4.1
    Matrix metalloproteinase 9 (gelatinase B, 92-kDa gelatinase, 92-kDa type IV collagenase)MMP931859_at10.6
    Secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1)SPP12092_s_at−4.718.5
    Zona pellucida glycoprotein 3 (sperm receptor)ZP339720_g_at−1.7
  • a BAY11-7802, pharmalogical inhibitor of NF-κB; LY294002, pharmalogical inhibitor of PI(3)K.

  • b BAY11-7802 and LY294002 were incubated with monocytes for 1 h prior to infection with HCMV.