TABLE 1.

Colocalization of RSV F proteins with CTxB and Tfna

ProteinOverlap coefficient (mean ± SD)P value (CTxB vs Tfn)b
CTxBTfn
SynF0.848 ± 0.010.552 ± 0.060.016
C550S mutant0.792 ± 0.010.607 ± 0.060.037
Δ555-5740.881 ± 0.050.528 ± 0.020.004
Δ551-5740.837 ± 0.030.511 ± 0.040.005
Δ550-5740.850 ± 0.010.386 ± 0.070.006
F/VSV G TM-CTD0.773 ± 0.040.432 ± 0.030.013
  • a Images of transfected A549 cells labeled for RSV F protein and CTxB or Tfn were acquired by confocal microscopy. Overlap between fluorescently labeled RSV F proteins and CTxB or Tfn signals was determined for three samples in each transfection group. For Manders coefficients of colocalization, 1 is high colocalization and 0 is low. The overlap coefficients of RSV F protein with nuclei stained with DAPI for select images ranged between 0.1 and 0.2.

  • b CTxB versus Tfn (column 4) refers to CTxB colocalization with RSV F protein (column 2) versus Tfn colocalization with RSV F protein (column 3).