TABLE 1.

Characteristics of DI- and DII-specific MAbs used in functional studies

AntibodyIsotypeWNV yeast reactivityaDENV-2 yeast reactivityEpitope(s)c
E18G2aDI-DIIDI-DIIDII-fl
E31G2aDI-DIIDI-DIIDII-fl
E53G2aDI-DIINRbDII-fl, DI-lr
E60G2aDI-DIIDI-DIIDII-fl
E65G2aDI-DIIDI-DIIDII-fl
E113G2aDI-DIINRDII-hi
E100G2aDI-DIINRDII-di
E101G2aDI-DIINRDI-lr
E48G2aDI-DIINRDII-ci
E121G2aDI-DIINRDI-lr
7H7G2aDI-DIINRDII-lr, DI-lr
7G5G2aDI-DIINRDI-li
E16G2bDIIINRDIII-lr
E34G1DIIINRDIII-lr
  • a DI-DII indicates binding to yeast expressing WNV or DENV-2 E DI-DII, with no binding to WNV or DENV-2 E DIII.

  • b NR, no reactivity to any of the DENV-2 yeast constructs.

  • c DII-fl, DII fusion loop; DI-lr, DI lateral ridge; DII-hi, hinge interface between DI and DII; DII-di, dimer interface; DII-ci, DII central interface; DII-lr, DII lateral ridge; DI-li, linker region between DI and DIII; DIII-lr, DIII lateral ridge.