TABLE 2.

Comparison of percentage of PBMC expressing p24-Ag to number of viral gag copies per 100 cellsa

VirusVirus dilution% of cells positive for p24-AgNo. of copies of gag DNA per 100 PBMCFold difference
BaL2.838.973.17
1:22.057.403.61
1:41.746.733.87
1:81.144.203.69
1:160.852.472.91
1:320.481.643.42
SF1623.8918.114.66
1:22.3013.285.78
1:41.466.974.78
1:80.945.365.71
1:160.522.785.36
1:320.241.405.88
  • a PHA- and IL-2-stimulated PBMC were exposed to serial dilutions of virus in medium containing indinavir. On day 2, aliquots were split and evaluated by flow cytometry for intracellular p24-Ag expression and by quantitative PCR for viral gag DNA. The MOI of undiluted virus was ∼1.0.