TABLE 1.

Growth of SW13 cell-passaged and parental JEV in IFN-α-R−/− mice inoculated i.v. with 105 PFU

Mouse group and days p.i.Virus titera in:
SerumSpleen LiverbBrainb
JnJsJnJsJnJsJnJs
Six-week-old mice
    14 × 105 <1005 × 105 8 × 105 1 × 103 2 × 103 2 × 103 1 × 103
3 × 105 <1007 × 105 1 × 105 1 × 104 2 × 103 2 × 103 1 × 103
4 × 105 <1007 × 105 7 × 104 2 × 104 2 × 103 5 × 103 <1 × 103
7 × 105 <1001 × 106 2 × 105 5 × 104 2 × 103 1 × 104 <1 × 103
    22 × 106 <1006 × 106 3 × 104 5 × 105 1 × 103 3 × 105 4 × 103
5 × 106 <1006 × 106 3 × 103 1 × 106 1 × 103 2 × 105 <1 × 103
    42 × 105 <1002 × 107 <1 × 103 2 × 106 1 × 103 2 × 109 <1 × 103
9 × 105 <1001 × 107 3 × 103 2 × 106 1 × 103 2 × 109 <1 × 103
Three-week-old mice
    1NTc <100NTNTNTNTNTNT
NT<100NTNTNTNTNTNT
    2NT1 × 103 NT9 × 106 NT2 × 104 NT3 × 103
NT400NT2 × 107 NT3 × 104 NT3 × 104
    4NT<100NT4 × 105 NT2 × 103 NT7 × 108
NT<100NT7 × 104 NT2 × 103 NT1 × 108
  • a Virus titers in tissues from individual animals were determined by plaque formation on Vero cells and are expressed as PFU per gram of tissue or PFU per milliliter of serum.

  • b Virus titers may have been underestimated due to the inhibitory effect of tissue homogenates at low dilutions on plaque formation (see Table 2).

  • c NT, not tested.