TABLE 3.

Organ distribution of radiolabeled virulent MVEAsp390 and attenuated MVEHis390 at 30 min after i.v. inoculationa

Mouse% cpm of input Infectivity
LiverSpleen Lungs Kidneys Blood
Asp390His390Asp390His390Asp390His390Asp390His390Asp390His390
15.612.00.31.00.40.70.51.027<0.5
25.812.00.41.70.50.70.51.022<0.5
  • a Six-week-old C57BL/6 mice were inoculated i.v. with 35S-labeled MVEAsp390 (7.2 × 104 cpm; 4× 107 PFU) or MVEHis390 (4.6 × 104 cpm; 6 × 105 PFU), and the livers, spleens, lungs, and kidneys were collected at 30 min p.i. for determination of virus load by scintillation counting. Blood was collected at the same time for determination of infectivity by plaque titration. Values are given as a percentage of input radioactivity or infectivity.