TABLE 1.

Infectivity of viruses in murine and human cells

MHV-2aFLS virusInfectivity (TCID50/ml)aRatiob
LR7 cellsHeLa cells
WT5.1 × 107≤63≥8.1 × 105
rec3.2 × 1072.5 × 10613
A4.6 × 108≤63≥7.3 × 106
AB5.3 × 108≤132≥4.0 × 106
AC8.5 × 1076.2 × 10614
AD1.9 × 109≤63≥3.0 × 107
Ac2.1 × 1082.2 × 105960
C6.8 × 106≤63≥1.1 × 105
  • a The viral infectivity in passage 2 stocks was determined by a quantal assay on LR7 (murine) and HeLa (human) cells, and their TCID50 values were calculated. The mean value of two independent recombinants is shown.

  • b The ratio of the TCID50 values on LR7 and HeLa cells is calculated.