TABLE 1.

Oligonucleotides used to introduce the intron into three different positions of the TGEV full-length cDNA

OligonucleotideSequencea
Long nameShort name
TGEV-8603-vsV15′-CCTAGTTTTCCTCTATGACTCACTCCC-3′
pRLi8826-5′-rsV25′-CCTTGATACTTACCATTGAACCTGTGTAGTAC-3′
pRLi8826-5′-vsI15′-CAGGTTCAATGGTAAGTATCAAGGTTACAAG-3′
pRLi8826-3′-rsI25′-CCATTCTGTAGTCAGCTTCTCCCTGTGGAGAGAAAG-3′
pRLi9466-3′-vsV35′-CCTTTCTCTCCACAGGTTCCAATTTTGAAGGAGAAATG-3′
TGEV-9759-rsV45′-CCTTGTTGAGTCTTACGATGC-3′
pRLi9466-5′-vsI35′-GTATACATGCATCACTTAGAACTTGGAAATGGCTCGCATGTTGGTAAGTATCAAGGTTACAAG-3′
pRLi9466-3′-rsI45′-CCTTCAAAATTGGAACCTGTGGAGAGAAAGGCAAAG-3′
TGEV-9291-vsV55′-GCTTGTTATGAAGGATGTC-3′
PRLi9596-5′-rsV65′-TGATACTTACCTTTCACCATTGATAAGTG-3′
PRLi9596-5′-vsI55′-AATGGTGAAAGGTAAGTATCAAGGTTACAAG-3′
PRLi9596-3′-rsI65′-TGACATCGATGTGTTTGTAACAAACCACCTGTGGAGAGAAAGGCAAAG-3′
  • a Bold letters indicate the sequence corresponding to the pRL-CMV intron.