TABLE 4.

Reactivity of antipeptide affinity-purified antibodies to synthetic peptides bound to MBS-coated ELISA plates

PeptideReactivitya for:
Eluted R4N-R1N columnEluted S2 columnEluted S2 + eluted R1NEluted S4 columnEluted P7b column
S1 (epitopes A and B) VLVNANNSSS SHFQSIYNC0.164 (± 0.012)0.269 (± 0.007)0.436 (± 0.006)0.007 (± 0.006)−0.008 (± 0.003)
S2 (epitope A removed, epitope B present) SGSGANNSSSSHFQSIYNC−0.004 (± 0.004)0.336 (± 0.005)0.351 (± 0.025)0.008 (± 0.000)0.007 (± 0.024)
S3 (epitopes A and B removed) SGSGANNSSSSGSGSIYNC0.001 (± 0.002)0.003 (± 0.004)0.000 (± 0.001)0.002 (± 0.001)−0.009 (± 0.005)
S4 (mimotope B) SHITSYHPAYFWC−0.007 (± 0.001)0.010 (± 0.021)NDc−0.005 (± 0.005)−0.010 (± 0.003)
R4N (mimotope A) ALVNIPISNNLAC0.540 (± 0.027)0.002 (± 0.002)ND0.010 (± 0.006)0.015 (± 0.007)
R1N (epitope A) VLVNSNNSSSSAC0.410 (± 0.024)0.000 (± 0.005)ND0.009 (± 0.001)0.005 (± 0.019)
Δ4R1N (epitope A removed) SGSGSN NSSSSAC0.005 (± 0.008)0.001 (± 0.003)ND0.004 (± 0.001)0.004 (± 0.009)
  • a Results are expressed as corrected OD at 405 nm (OD for tested peptide − OD for irrelevant peptide P7) (± standard deviation).

  • b Irrelevant peptide P7 (QRAYLELPPWPP).

  • c ND, not done.