Comparison of Huh-7 subclones for transfectability and infectibility by HEV

SubcloneTransfection efficiencya (GFP intensity)No. of cells infectedb
10-34+ (3+)273
10-71+ (2+)5
10-81+ (2+)17
10-92+ (2+)48
10-104+ (4+)42
10-111+ (2+)49
13-13+ (3+)9
  • a Cells were transfected with an HEV replicon encoding GFP, and the proportion of cells producing GFP and the GFP fluorescence intensity were estimated on a scale of 0 to 4.

  • b Five days after cultures were incubated with a human stool suspension containing HEV, the cells were stained with anti-ORF2 protein for immunofluorescence microscopy, and the number of cells containing ORF2 capsid protein was tallied.