TABLE 1.

Enhanced virus production in the absence of PML in vitro

MEF genotypeaVSVLCMV-WE
MOIVirus productionb (PFU/ml)MOIVirus productionb (PFU/ml)
PML−/−56.5 × 1065 × 10−28 × 104
PML+/+52.5 × 1065 × 10−22 × 104
PML−/−0.52.3 × 1075 × 10−33.6 × 106, 1.6 × 106, 4.5 × 106
PML+/+0.52.4 × 1065 × 10−34 × 105, 5 × 105, 5.4 × 105
PML−/−5 × 10−26.5 × 1061 × 10−32 × 107
PML+/+5 × 10−21.2 × 1061 × 10−39.5 × 106
PML−/−1 × 10−24.5 × 106, 4.7 × 1065 × 10−41.5 × 106
PML+/+1 × 10−27 × 105, 7.1 × 1055 × 10−44 × 105
PML−/−5 × 10−38 × 1055 × 10−54.4 × 105
PML+/+5 × 10−34.2 × 1055 × 10−54 × 104
  • a A total of 5 × 105 PML−/− or PML+/+ control MEFs for each experimental condition were inoculated with 2.5 × 106, 2.5 × 105, 2.5 × 104, 5 × 103, or 2.5 × 103 PFU of VSV or with 2.5 × 104, 2.5 × 103, 5 × 102, 250, or 25 PFU of LCMV-WE corresponding to the indicated MOIs.

  • b Each value represents viral titers determined after 24 (VSV) or 48 (LCMV) h by transferring pooled supernatants of eight individual cultures onto Vero (VSV) or MC57G (LCMV) cell monolayers.