TABLE 2.

N-glycosylation sites in HIV-1 gp120 deleted in mutant HIV-1(IIIB or NL4.3) strains that emerged under escalating CV-N exposurea

N-glycosylation sites in HIV-1 gp120bGlycan naturecMutation(s) detected at CV-N concn (μg/ml) (selection series, code no.)
0.1 (1, 107-10)1 (1, 113-24)1 (2, 108-4)2 (2a, 113-14)2 (2b, 113-15)0.1 (3, 107-9)1.5 (3, 113-23)
136NDT138H/C136N/K
160NIS162H/C162S/N162S/N162S/N
230NKT232M232T/M232T/M
332NIS334M332I334N334N334N
339NNT341M339S339S339S339S341A
386NST388M386D
392NST394M392N/T392N/T
448NIT450M448N/Y448S448S448S
  • a The wild-type virus used for the drug resistance selections was HIV-1(IIIB) for selection series 1 and 2 and HIV-1(NL4.3) for selection series 3.

  • b Numbering is according to the method of Kwong et al. (19). Boldface type indicates the nature of the amino acids in the glycocylation motifs.

  • c High-mannose type (M) versus complex/hybrid type (C/H) glycans according to Leonard et al. (21) and Gallaher et al. (16).