TABLE 1.

Oligonucleotides used for electrophoretic mobility shift assay

OligonucleotideSequenceaPositionb
wt15′-TACCACAAACAACCTCAGGTTTTCCTGCGACGC-3′69602-69634
mut15′-TACCACAAACAACCTTTAATTTTCCTGCGACGC-3′
wt25′-GTATTCTGGATTCCAAACGTGCCCAGCGGTACCCAA-3′70450-70485
mut25′-GTATTCTGGATTCCAATTAATCCCAGCGGTACCCAA-3′
wt35′-ACTCCAACCGTCTCACGTCCGAGGTAATGTGCTC-3′70707-70740
mut35′-ACTCCAACCGTCTCTTAACCGAGGTAATGTGCTC-3′
wt45′-GTAGCTGGGTGGCAATGACACGTCCCCTTTAAAAAGTC-3′71502-71539
mut45′-GTAGCTGGGTGGCAATGACTTAACCCCTTTAAAAAGTC-3′
wt55′-GGAGAGTTAGGGACGTGCTGATTATGTGGAC-3′71819-71849
mut55′-GGAGAGTTAGGGTTTAACTGATTATGTGGAC-3′
wt65′-GTTTAGAGCGGGTACGTGGCAGTCTGGATTGA-3′72396-72427
mut65′-GTTTAGAGCGGGTTTTAAGCAGTCTGGATTGA-3′
  • a Only the sequences of the sense oligonucleotides are shown. For the electrophoretic mobility shift assay, the complementary probes were used. The putative hypoxia response elements are shown in boldface type, and the mutations are underlined.

  • b The positions are relative to the KSHV genome.