TABLE 1.

Characteristics, MPX-associated symptoms, and viremia in mock-immunized monkeys and those immunized with Lister or LC16m8

GroupIDaVaccinationSexb (wt [g]MPXV challenge strain (route,c dose [PFU])Virus isolationd (no. of plaques at the indicated day of collection)MPX-associated symptoms
Symptom(s) at the site of MPXV inoculationNo. of papulovesicular lesionsOutcome
Expt 1
    IN-Naïve4595MockF (2,500)Liberia (IN, 106)Positive (20 at day 4, 10 at day 6, 10 at day 8)Rhinorrhea10Survival
4596F (2,320)Positive (10 at day 6, 10 at day 13)Rhinorrhea16Survival
    IN-Lister4597ListerF (2,360)NegativeNone0Survival
4598F (2,580)NegativeNone0Survival
4599F (2,700)NegativeNone0Survival
    IN-LC16m84600LC16m8F (2,650)NegativeNone0Survival
4601F (2,800)NegativeNone0Survival
4602F (2,700)NegativeNone0Survival
Expt 2
    SC-Naïve4651MockF (2,560)Zr-599 (SC, 106)Positive (4 at day 3, 5 at day 7, 33 at day 9, 30 at day 14)Erythema, papulovesicles, ulcer390Death
4653M (3,100)Positive (176 at day 3, 18 at day 7, 3 at day 9, 23 at day 11, 9 at day 14, 3 at day 18)Erythema, papulovesicles, ulcer1,150Death
    SC-Lister4575ListerF (2,980)NegativeNone0Survival
4576F (3,100)NegativeNone0Survival
    SC-LC16m84577LC16m8F (2,640)Positive (1 at day 3)Erythema0Survival
4525F (2,180)Positive (7 at day 3, 1 at day 6)Erythema, papulovesicles, ulcer0Survival
4526F (2,730)NegativeErythema, papulovesicles, ulcer0Survival
  • a ID, monkey identification number.

  • b F, female; M, male.

  • c IN, intranasal inoculation; SC, subcutaneous inoculation.

  • d Positive or negative, MPXV was or was not isolated, respectively, during the observation period from challenge to sacrifice. In experiment 1, the intranasal inoculation model, virus isolation was attempted from aliquots of 106 purified PBMCs collected from monkeys on days 0, 2, 4, 6, 8, 10, 13, 16, and 20 after virus challenge. In experiment 2, the subcutaneous inoculation model, virus isolation was attempted from the buffy coat fractions collected from 4-ml aliquots of total peripheral blood collected on days 0, 3, 7, 9, 11, 14, and 18.