TABLE 3.

Summary of all peak T-cell responsesa to pool 90 and positive responses to pool 9 (in parentheses) induced by vaccination

Vaccination group (n = 8)kSubject no.Assay and T-cell response
Cultured IFN-γ ELISPOT[3H]thymidine proliferationEx vivo IFN-γ ELISPOTIL-7-IL-15 IFN-γ ELISPOTCFSE proliferationIFN-γ WB ICS
pTHr.HIVA-MVA.HIVA17,769b (5,925)224.2959 (279)2165 (718)0.560.46 (0.18)c
210,431d (475)11.6103166e0.070.01
33,206b44.4514NDfND0.02
44,181b7.3336g860.030.01
51,463b8.5311280.010.03
6388b4.2830960.000.02
7413b0.934180.010.01
81,769b1.595130.010.01
MVA.HIVA92,494 (906)0.3628114h (165)ND0.01
10(1,250)0.15−1−3NDND
11(356)0.97015ND0.00
123,8380.556NDND0.00
13280i1.01016ND0.00
1400.33−18ND0.00
150j0.69−131ND0.00
16−250.171116ND0.00
  • a Background-subtracted responses are shown. Unless otherwise indicated, responses shown are from week 9 in the pTHr.HIVA-MVA.HIVA group and week 1 in the 2× MVA.HIVA group. Positive T-cell responses as defined in Table 2 are in boldface.

  • b Positive T-cell response from week 10 (2 weeks post-MVA.HIVA). No assays were performed at week 9.

  • c Peak pool 9-specific T-cell response detected at week 10.

  • d Positive T-cell response from week 12 (4 weeks post-MVA.HIVA). There were insufficient cells to perform the assay at week 10.

  • e A value of 116 SFC/106 cells does not equal a positive response for this subject, because 116 SFC is less than 4× mock at this time point (see Table 2).

  • f ND, no data were available for pool 90-specific T-cell responses at week 9 or week 1. No positive responses were recorded at any other time points.

  • g A value of 6 SFC/106 cells is a positive response, because this subject had a response of >30 SFC/106 cells to two peptide pools (see Table 2).

  • h A value of 114 SFC/106 cells is a positive response, because this subject had a response of >93 SFC/106 cells to two peptide pools (see Table 2).

  • i Pool 1 response is shown. The greater proportion of the pool 90 response was attributable to pool 10 and therefore was excluded from the definition of a vaccine-induced T-cell response (see Table 2).

  • j Prevaccination data were available for pool 9 STCL only.

  • k No positive responses were recorded for the placebo group (subjects 17 to 24).