TABLE 1.

Oligonucleotide primer and probe sequences for amplification of porcine genes used in real-time RT-PCR

Gene typePrimer and probe setsSequenceGenBanka
GAPDHPorcine GAPDH-327Fb5′-CGTCCCTGAGACACGATGGT-3′AF017079
Porcine GAPDH-380Rc5′-CCCGATGCGGCCAAAT-3′
Porcine GAPDH-348Td5′-AAGGTCGGAGTGAACG-3′
Mx1Porcine Mx1-803Fb5′-GAGGTGGACCCCGAAGGA-3′M65087
Porcine Mx1-859Rc5′-CACCAGATCCGGCTTCGT-3′
Porcine Mx1-824Td5′-AGGACCATCGGGATC-3′
OASPorcine OAS-889Fb5′-CTGTCGTTGGACGATGTATGCT-3′AJ225090
Porcine OAS-954Rc5′-CAGCCGGGTCCAGAATCA-3′
Porcine OAS-919Td5′-TCAAGAAACCCAGGCCT-3′
PKRPorcine PKR-968Fb5′-GGAAGAAAACAAACACAGCTTGAA-3′AB104654
Porcine PKR-1048Rc5′-CCAAATCCACCTGAGCCAATT-3′
Porcine PKR-994Td5′-CCAGGTT TGTCGAAGAT-3′
pIFNα set#1Porcine IFNα-236Fb5′-TGGTGCATGAGATGCTCCA-3′M28623
Porcine IFNα-290Rc5′-GCCGAGCCCTCTGTGCT-3′
Porcine IFNα-256Td5′-CAGACCTTCCAGCTCT-3′
pIFNα set#2Porcine IFNα-449Fb5′-TCACCCTCTATCTGCAAGAGAAGA-3′M28623
Porcine IFNα-514Rc5′-TGACTTCTGCCCTGACGATCT-3′
Porcine IFNα-478Td5′-AGCCCTGTGCCTG-3′
pIFNβPorcine IFNβ-11Fb5′-AGTGCATCCTCCAAATCGCT-3′M86762
Porcine IFNβ-69Rc5′-GCTCATGGAAAGAGCTGTGGT-3′
Porcine IFNβ-32Td5′-TCCTGATGTGTTTCTC-3′
  • a NCBI GenBank accession code.

  • b Forward primer.

  • c Reverse primer.

  • d TaqMan FAM-MGB probe.