TABLE 2.

Summary of AP-binding and trafficking phenotypesdd

NefY3Ha with hemicomplexesGST pull-downStabilizationbCD4 down-regulationInternalization as CD8-NefcInvariant chain up-reg.DC-SIGN up-reg.Tf uptake block
AP-1AP-3AP-1AP-3AP-1AP-3
Wild type+++++++++++
LL/AA
E160A++IntInt
E160GInt+
E160KInt
N161G++++++++IntInt+
T162G++IntInt
S163GInt+++IntIntInt
  • a Y3H, yeast three-hybrid.

  • b Stabilization on endosomes in brefeldin-treated cells.

  • c Inverse of surface expression.

  • d +, positive; −, negative; Int, intermediate; reg, regulation.