TABLE 2.

Frequency of substitutions in envelope clones from H445 tissues, SIVsmH445 virus stock and sequential plasma samples from H635

SubstitutionFrequencya (wpi, no. of clones analyzed) in clones from:
H445 tissues (16, 14)SIVsmH445 stock (—, 10)H635 plasma
1, 62, 94, 109, 16
T132Δ0 (0)0 (0)16.7 (1)0 (0)10.0 (1)0 (0)
S154P0 (0)0 (0)0 (0)0 (0)10.0 (1)6.3 (1)
N158D35.7 (5)10.0 (1)0 (0)11.1 (1)0 (0)93.8 (15)
S160N/G35.7 (5)0 (0)0 (0)0 (0)0 (0)0 (0)
(N158D or S160N/G)b64.3 (9)10.0 (1)0 (0)11.1 (1)0 (0)93.8 (15)
N175S or T177Ab0 (0)0 (0)0 (0)11.1 (1)10.0 (1)0 (0)
I185T/R14.3 (2)0 (0)0 (0)0 (0)0 (0)0 (0)
N215D or S217Nb7.1 (1)30.0 (3)0 (0)0 (0)0 (0)0 (0)
N298D or T300A/Ib7.1 (1)10.0 (1)0 (0)11.1 (1)0 (0)0 (0)
R315K14.3 (2)20.0 (2)0 (0)0 (0)0 (0)0 (0)
V325G/A7.1 (1)0 (0)0 (0)0 (0)10.0 (1)0 (0)
P337T/S/H/L92.9 (13)10.0 (1)0 (0)0 (0)20.0 (2)93.8 (15)
E340K85.7 (12)0 (0)0 (0)0 (0)20.0 (2)93.8 (15)
R348W92.9 (13)30.0 (3)0 (0)11.1 (1)60.0 (6)100.0 (16)
G386R28.6 (4)0 (0)0 (0)0 (0)0 (0)6.3 (1)
D388N/G50.0 (7)10.0 (1)0 (0)0 (0)0 (0)93.8 (15)
W395R0 (0)10.0 (1)0 (0)0 (0)10.0 (1)0 (0)
Q419R7.1 (1)0 (0)16.7 (1)0 (0)0 (0)0 (0)
S421G/N7.1 (1)10.0 (1)0 (0)0 (0)0 (0)0 (0)
P430T/S/L/R57.1 (8)10.0 (1)0 (0)0 (0)20.0 (2)100.0 (16)
Q432R7.1 (1)10.0 (1)0 (0)0 (0)0 (0)0 (0)
H450R/Y7.1 (1)0 (0)0 (0)0 (0)0 (0)6.3 (1)
E477K57.1 (8)0 (0)0 (0)0 (0)0 (0)12.5 (2)
D519N35.7 (5)0 (0)0 (0)0 (0)40.0 (4)93.8 (15)
  • a Percentage of substitutions in total clones; the number of clones with substitutions is shown in parentheses. All the substitutions which are observed in more than one clone are shown here. Boldface indicates substitutions dominant in H635 at 9 wpi.

  • b Loss of potential N-linked glycosylation sites.