TABLE 1.

Envelope glycoprotein and V1, V2, and V3 variable loop reactivity of the sera from immunized guinea pigsa

ImmunogenGroupAdjuvantAnimalEnd-point titerb
gp120gp120ΔV12V1V2 poolV1(a02) peptideV3 peptide
YU2gp120
ARibi40+++++++++++++
41++++++++++
42+++++++++++++
43++++++++++
BAS01B44+++++++++++++++++
45++++++++++++++
46++++++++++++++++++
CAS02A52++++++++++++++++++
53+++++++++++++++++++
54++++++++++++++++++++
55+++++++++++++++++
DAS0348+++++++++++++
49+++++++++++++
50++++++++++++/−
51++++++++++++
YU2gp140(−/GCN4)
ERibi56+++++++++++++++++
57++++++++++++++++
58+++++++++++++++
59++++++++++++
FAS01B60++++++++++++++++
61++++++++++++++
62+++++++++++++++++
63+++++++++++++
GAS02A68+++++++++++++
69+++++++++++++++++++++
70++++++++++++++++
71++++++++NDc+++++++
HAS0364+++++++++++++++
65++++++++++++
66++++++++++++
67+++++++++++++
  • a Sera were collected after the fourth inoculations, with preimmune sera as a negative control in the assay.

  • b End-point ELISA titers were defined as the last reciprocal serum dilution at which the optical density signal was greater than twofold over the signal detected with the preimmune serum. Symbols for the end-point titers are as follows: +/−, 2.5 × 103; +, 1.25 × 104; ++, 6.25 × 104; +++, 3.13 × 105; ++++, 1.5 × 106; +++++, 7.5 × 106.

  • c ND, not determined.