TABLE 2.

Neutralization of YU2 virus and V1, V2, and V3 loop peptide adsorptions of the sera elicited by YU2 gp120 and gp140(−/GCN4)

ImmunogenGroupAdjuvantAnimalNeutralization activity (%)% Neutralization with peptides (% inhibition)a
V3 scrambled peptideV3 peptideV1V2 poolV1V2 pool 1bV1 a02V1V2 pool 3c
YU2 gp120
ARibi40d
41
425757 (0)48 (15)57 (0)0 (100)57 (0)
43
BAS01B4484 ± 6e82 (2)84 (0)33 (61)24 (71)
45
4684 ± 882 (2)82 (2)0 (100)0 (100)
CAS02A524444 (0)31 (30)40 (10)0 (100)44 (0)
539191 (0)88 (3)89 (2)0 (100)89 (2)
545252 (0)28 (47)52 (0)0 (100)40 (24)
555252 (0)41 (22)44 (15)0 (100)44 (15)
DAS03487166 (7)50 (30)46 (35)0 (100)38 (47)
498786 (1)84 (3)80 (8)28 (68)77 (11)
504030 (24)39 (3)28 (29)0 (100)40 (0)
517263 (13)63 (13)68 (6)42 (42)60 (14)
YU2 gp140(−/GCN4)
ERibi56
5732
58
5953
FAS01B6099 ± 299 (0)99 (0)77 (22)94 (5)80 (19)92 (7)
618377 (7)63 (24)83 (0)26 (69)83 (0)
6299 ± 198 (1)99 (0)73 (26)97 (2)76 (23)98 (1)
638281 (1)68 (17)67 (18)45 (45)81 (1)
GAS02A689898 (0)98 (0)98 (0)98 (0)98 (0)
699999 (0)95 (4)96 (3)50 (50)95 (4)
709591 (4)93 (2)94 (1)67 (29)92 (3)
719796 (1)92 (5)96 (1)60 (38)97 (0)
HAS03649797 (0)97 (0)97 (0)49 (50)97 (0)
659696 (0)91 (5)92 (4)22 (77)93 (3)
667067 (5)64 (8)67 (4)12 (83)21 (70)
679695 (1)90 (6)84 (13)
  • a Percent inhibition was calculated by comparing the neutralization activity in the presence and absence of peptides, and the values are shown in parentheses and italics.

  • b V1V2 pool 1 consists of peptides YU2a01 and YU2a03.

  • c V1V2 pool 3 consists of peptides YU2a04, -a05, -a06, and -a07.

  • d Animal sera that contained a low level of neutralization activity were not analyzed (indicated by underlining). If a value is not shown for neutralization or peptide inhibition, then the analysis was not determined due to the limitation of available serum.

  • e For sera in which the peptide inhibition assays were performed in replicate assays (three or greater), a standard deviation value is shown.