TABLE 1.

Characteristics of SHIV-89.6P-infected Macaca nemestrina

GroupAnimal no.Vaccine groupViral load (RNA copies/ml plasma at 32 wpi)CD4+ T cells (cells/μl at 32 wpi)Ratio of dN/dSa% Divergence at wk 32b% Diversity at wk 32Avg no. of PNGsc
Minimally divergentK97107Control2.8 × 1021,1550.30.30.622.0
K98099DNA-vaccinia virus1.2 × 1036010.20.3*0.521.9
L97191DNA-vaccinia virus2.5 × 105730.50.3*0.521.3
J97168Vaccinia virus-DNA5 × 1011,3720.30.40.721.7
DivergentT98098DNA-protein1.6 × 105400.80.9*1.220.2d
A98069DNA-DNA1.2 × 1041411.31.2*1.622.2
J98071Control1.4 × 105291.81.4**1.323.7
J97156DNA-protein5.6 × 104562.21.8**1.624.9
K97246DNA-DNA1.6 × 105112.41.91.524.1
T98108DNA-protein5.9 × 10452.01.9**1.024.3
L98152Vaccinia virus-DNA5 × 1011,8202.91.9**1.421.1
  • a Average ratio within the proviral clones of nonsynonymous change to synonymous change, calculated using PAML codeml.

  • b Average percent divergence of each animal's cloned quasispecies away from the inoculum. *, Mann-Whitney P value of <0.05; **, P value of <0.001.

  • c Average number of PNGs per env region from V1 to gp41. Note that N88 is not included in this region and therefore not represented in the average but is likely well conserved.

  • d T98098 sequence reads did not cover the N130 and N136 motifs, which were well conserved in the other macaques. Therefore, the average is likely a low estimate.