TABLE 5.

Viremia following challenge with 106 MID50 DENV-4 in rhesus monkeys passively administered IgG 5H2 ΔDa

MonkeyIgG 5H2 ΔD (0.9 mg/ml) administrationViremiaPeak virus titer (FFU/ml)b
Onset dayDuration (days)
RH69026230
RH6702510
RH692+66801
RH688+642001,2
RH693+452102
RH685+8210
  • a Rhesus monkeys that received 0.9 mg/kg of IgG 5H2 ΔD (approximately 300 PRNT50/ml) intravenously were challenged subcutaneously with 1 × 106 MID50 DENV-4 24 h later. Control monkeys (RH690 and RH670) that received the diluent PBS were similarly challenged. Monkey serum samples obtained on days 1 to 12 postchallenge were analyzed for viremia by focus-forming assay.

  • b Putative neutralization escape mutant viruses recovered had Lys174-Arg (1) and Lys174-Glu (2) mutations.