TABLE 1.

Summary of mutation frequencies of HTNV vRNA in the absence or presence of various chemical treatments

Treatment of HTNV-infected Vero E6 cellsNo. of cDNAs examinedNo. of nt analyzed% of cDNAs with mutations% of C→U or G→A mutations of totalMutation frequencyb
C→U, G→AU→C, A→GOtherscTotal
No treatment85105,31511.836.40.40.20.51.0
RBV
    41 μM87107,79323.077.31.6¶0.20.32.0
    82 μM88109,03236.453.81.9§0.80.83.6§
    123 μM170210,63034.773.42.8§0.40.63.8§
    164 μM91112,74941.875.03.5§0.60.54.6§
    246 μM182225,49844.066.03.0§0.70.94.6§
MPA, 15.6 μM95117,70515.826.30.40.70.51.6
RBV + MPAa92113,98842.477.04.1§0.70.55.4§
TIA, 154 μM90111,51018.950.00.80.40.81.6
SEL, 130 μM93115,22718.335.00.60.31.11.7
  • a Cotreatment of 41 μM RBV with 3.1 μM MPA.

  • b Mutations per 10,000 nt sequenced. §, P < 0.001; ¶, P < 0.01 (Student's t test).

  • c Any mutations other than transition mutations, including frameshift.