TABLE 4.

Differential gene expression in MVA/NYVAC-infected DC compared to that in MVA/NYVAC-infected HeLa cellsa

DescriptionGenBank accessionGeneFold change
MVANYVAC
Immune response, inflammatory response
    Small inducible cytokine A3NM_002983SCYA366.8110.63
    IFN-induced protein with tetratricopeptide repeats 4NM_001549IFIT432.5210.87
    TNF (TNF superfamily, member 2)NM_000594TNF31.405.60
    IL-8NM_000584IL820.5331.45
    V-fos FBJ murine osteosarcoma viral oncogene homologNM_005252FOS20.491.75
    Guanylate binding protein 5NM_052942GBP515.21
    CD83 antigen (activated B lymphocytes, immunoglobulin superfamily)NM_004233CD8314.5312.41
    IFN-β1, fibroblastNM_002176IFNB113.704.52
    GRO2 oncogeneNM_002089GRO213.211.64
    Pre-B-cell colony-enhancing factorNM_005746PBEF8.0113.38
    MDA5NM_022168MDA56.66
    Nuclear factor of kappa light polypeptide gene enhancer in B cells 1 (p105)NM_003998NFKB15.7012.59
    RNA helicaseNM_014314RIG-I5.42
    IFN-α-inducible protein (clone IFI-6-16)NM_022873G1P34.692.77
    IRF-7NM_004031IRF74.243.37
    IFN-induced protein with tetratricopeptide repeats 1NM_001548IFIT13.888.63
    IFN-γ-inducible protein 30NM_006332IFI303.515.85
    Myxovirus (influenza virus) resistance 1, IFN-inducible protein p78 (mouse)NM_002462MX13.45
    Small inducible cytokine A4NM_002984SCYA414.94
Apoptosis
    TNF-α-induced protein 3NM_006290TNFAIP318.2420.04
    Apolipoprotein ENM_000041APOE15.454.58
    TNF receptor-associated factor 1NM_005658TRAF12.434.92
    Death-associated protein 6NM_001350DAXX−1.226.17
    IL-1βNM_000576IL1B5.49
    Cathepsin BNM_001908CTSB11.43
Cell cycle, signaling
    Carcinoembryonic antigen-related cell adhesion molecule 6M18216CEACAM678.4710.14
    Endothelial PAS domain protein 1NM_001430EPAS111.217.29
    LeupaxinNM_004811LPXN9.597.45
    Fibrinogen, gamma polypeptideNM_021870FGG7.7913.30
    Prostaglandin E receptor 1 (subtype EP1), 42kDNM_000955PTGER15.7610.54
    Mitogen-activated protein kinase 6NM_002748MAPK65.338.48
    Wingless-type MMTV integration site family, member 10BNM_003394WNT10B4.256.44
    PleckstrinNM_002664PLEK11.08
    CD53 antigenNM_000560CD539.48
    RAP1B, member of RAS oncogene familyNM_015646RAP1B15.26
  • a Both cell types were infected with MVA or NYVAC (5 PFU/cell), and total RNA was extracted at 6 hpi and processed for microarray and data analysis (see Materials and Methods and Results). FBJ, Finkel-Biskis-Junkins; PAS, Per-Arnt-Sim.