TABLE 3.

Expression levels of selected genes by real-time RT-PCRa

Gene productFold change by real-time RT-PCR
MVA vs mockNYVAC vs mock
DC 1DC 2DC 3DC 1DC 2DC 3
TNF36.6325.4544.322.234.954.48
IFN-β43.8628.8727.762.154.981.85
ISG153.824.623.812.152.272.26
IL-127.915.897.982.081.981.79
IL-71.780.902.071.271.550.81
IL-62.012.363.031.161.412.54
IFN-γ1.451.821.41.190.981
NFκ22.023.053.641.682.062
OASL27.6637.6626.632.131.430.519
ATF-31.962.191.983.513.753.91
H2AFY1.441.891.983.730.750.91
ADORAA20.7141.183.61.281.180.63
  • a MDDC from three donors (DC1, DC2, and DC3) were mock, MVA, or NYVAC infected and processed for RT-PCR.