TABLE 2.

Genes targeted by more than two distinct siRNAs suppressing RSV infection

Gene and primary functionaNumber of siRNAs giving significant (P < 0.05)b reductionReported function and other characteristic(s) of product
Clathrin-coated pit formation
    AP1B13Subunit of clathrin-associated adaptor protein complex 1
    AP2A22Subunit of clathrin-associated adaptor protein complex 2
    CLTB2Clathrin light chain B subunit; required for clathrin-coated pits
    DAB22Adapter protein that couples receptors into clathrin-coated pits
    ITSN23Regulates formation of clathrin-coated vesicles
    SYNJ12Localized to clathrin-coated vesicles and binds EPS15
Endocytosis
    DNM32Dynamin 3; required for pinching off endocytic vesicles from the plasma membrane
    RAB5A3Required for early endosome formation
    RAB5B3As for Rab5A
    RAB5C2As for Rab5A
    GAF12RAB11-interacting protein
    RAB11A2Important in recycling endosomes
    SYT12Involved in endocytosis
Actin polymerization
    ARPC1B3Part of the ARP2/3 complex implicated in the control of actin polymerization
    PAK12Regulates actin through Rac1
    WASF22Involved in signaling through actin
  • a The genes listed are those targeted by two or more single-siRNA duplexes that suppressed RSV infection signficantly (P < 0.05) at a dosage of 6 pmol per well of a 96-well plate containing 104 cells.

  • b Statistical significance was determined by one-way analysis of variance using the Tukey-Kramer posttest at a 95% confidence interval. The experiment was repeated twice, with similar outcomes.