TABLE 1.

Calculated number of cells which can be infected before the majority of each vector becomes unstable

VectorHEK293NIH 3T3U87-MG
Cycle no.aNo. of eGFP-positive cellsbCycle no.No. of eGFP-positive cellsCycle no.No. of eGFP-positive cells
ACE-GFP≥21≥5.5 × 1052111.2 × 1046206.4 × 1023
ACEU3-GFP159.9 × 102451.1 × 101442.2 × 1011
Akv(B)4070A-eGFP151.7 × 1016112.9 × 1021103.3 × 108
  • a Serial infection cycle number at which more than 50% of infected cells no longer express detectable levels of eGFP.

  • b Theoretical maximum number of cells to which a functional eGFP-expressing vector has been propagated by this time point (cycle).