TABLE 7.

HSV-1 WT and tk mutants detected in individual neurons from a ganglion of patient 708

Composition of virus population in single neurons (% of total no. of neurons)Single neurona identificationNo. of clones sampledNo. of clonesb with:% of mutant clones
7-G (WT) sequence1-G del1-G ins2-G ins3-G ins
Both WT and mutant clones insn-11612425
    each neuron (42.9)sn-2171616
sn-31612425
sn-42171467
sn-5245116279
sn-6171615.9
WT only in each neuron (28.6)sn-713130.0
sn-8660.0
sn-923230.0
sn-1010100.0
Mutants only in each neuron (28.6)sn-111313100
sn-1221183100
sn-1322220100
sn-141019100
  • a Single neurons were retrieved from LT9 DRG tissue sections by LCM.

  • b del, deletion; ins, insertion.