TABLE 5.

Determining the mutation rate during PCR subcloning of HSV-1 tk plasmid clones from patient 708 with nine-G sequences in the seven-G homopolymer region

PCR templateNo. of clones sampledNo. of clones with:% of mutant clones
9-G sequence9- and 8-G sequences8-G sequence7-G sequence
Topo143-2.7322811213
Topo143-4.1112901225
Total443712416