RNA content of retroviral particlesa

SampleCell lineNo. of samplesng RNA/ml supernatant ± SDpg RNA/ng Gag ± SDRNA:Gag molecule ratio (bp)
Ψ PR293T418 ± 4.038 ± 78
PR293T344 ± 2.036 ± 147
Mock293T31.1 ± 0.1
  • a RNA was extracted from particles as described in Materials and Methods and quantitated using Quant-IT Ribogreen. The amount of MLV Pr65Gag or HIV-1 Pr55Gag was estimated by semiquantitative Western analysis as described in Materials and Methods.