TABLE 2.

Cell numbers in T-cell subsets of peripheral lymphoid organs from Tg mice expressing different Nef alleles

Mouse lineaAbsolute cell no. (106)bCD4/CD8 ratioMean fluorescence (%)c of CD4
Total LNCD4CD8
Non-Tgd21.5 ± 5.510.6 ± 2.54.3 ± 0.82.5 ± 0.4100
    AD-93
        F11581720.1 ± 18.99.8 ± 8.03.0 ± 2.53.1 ± 0.2100
        F1158208.4 ± 5.7e1.8 ± 1.2e2.2 ± 1.6f1.0 ± 0.4e65 ± 10f
    032an
        F955819.7 ± 5.2f2.9 ± 2.0e1.6 ± 0.8e1.8 ± 0.3f89 ± 6
        F955827.8 ± 5.4e3.0 ± 2.6e1.6 ± 1.6f1.9 ± 1.185 ± 9
    039nm
        F755815.8 ± 3.0e0.8 ± 0.3e1.1 ± 0.3e0.7 ± 0.1e25 ± 8e
        F5144412.5 ± 7.0f2.4 ± 1.1e2.1 ± 0.6e1.1 ± 0.2e84 ± 16
    JR-CSF
        F1049956.6 ± 4.0e1.4 ± 1.2e1.9 ± 0.8e0.5 ± 0.2e71 ± 16f
        F10499615.3 ± 5.03.0 ± 1.4e6.1 ± 2.50.5 ± 0.2e11 ± 3e
    YU10x
        F9275111.3 ± 2.0e5.3 ± 1.0e3.1 ± 0.6f1.7 ± 0.2e97 ± 3
        F9275412.9 ± 5.5f6.2 ± 1.82.8 ± 0.9f2.1 ± 0.294 ± 6
    SF-2
        F927485.9 ± 4.0e0.3 ± 0.2e0.9 ± 0.1e0.3 ± 0.2e44 ± 26e
        F9274920.3 ± 15.09.3 ± 6.04.1 ± 3.82.7 ± 0.699 ± 9
    T71R
        F10137611.5 ± 3.0f5.4 ± 1.0e1.9 ± 0.5e2.8 ± 0.498 ± 2
        F1013749.3 ± 2.0e3.7 ± 1.0e2.4 ± 0.4e1.5 ± 0.3e65 ± 20f
        F10137128.4 ± 5.514.1 ± 3.05.9 ± 1.03.0 ± 0.395 ± 7
        F867078.8 ± 1.5e1.3 ± 0.3e2.2 ± 0.3e1.0 ± 0.1e21 ± 2e
Non-Tgd19.5 ± 3.59.3 ± 2.54.3 ± 1.02.2 ± 0.4100
    NL4-3(WT)6.3 ± 3.6e1.2 ± 0.5e1.7 ± 0.9e0.6 ± 0.1e24 ± 11e
  • a FACS analysis was performed on 4 to 10 mice (1.5 to 4 months old) for each Tg line, including the positive control CD4C/HIV-NefNL4-3(WT) Tg line. Data from Tg mice were always compared to those of their littermates done the same day.

  • b The absolute cell number was calculated by multiplying the total number of cells in each organ by the percentage of each cell subset in this organ.

  • c The mean fluorescences for CD4 were obtained by calculating the ratio of CD4 staining in Tg mouse thymuses relative to that of non-Tg mouse thymuses (100%) done in the same experiment, the same day. Mean values were then calculated with the values for each line.

  • d The non-Tg control values were obtained by pooling the results of all non-Tg littermates from different lines.

  • e Significant decrease (P < 0.01) according to Student's t test.

  • f Significant decrease (P < 0.05) according to Student's t test.