Mouse line^{a} | Absolute cell no. (10^{6})^{b} | CD4/CD8 ratio | Mean fluorescence (%)^{c} of CD4 | ||
---|---|---|---|---|---|

Total LN | CD4 | CD8 | |||

Non-Tg^{d} | 21.5 ± 5.5 | 10.6 ± 2.5 | 4.3 ± 0.8 | 2.5 ± 0.4 | 100 |

AD-93 | |||||

F115817 | 20.1 ± 18.9 | 9.8 ± 8.0 | 3.0 ± 2.5 | 3.1 ± 0.2 | 100 |

F115820 | 8.4 ± 5.7^{e} | 1.8 ± 1.2^{e} | 2.2 ± 1.6^{f} | 1.0 ± 0.4^{e} | 65 ± 10^{f} |

032an | |||||

F95581 | 9.7 ± 5.2^{f} | 2.9 ± 2.0^{e} | 1.6 ± 0.8^{e} | 1.8 ± 0.3^{f} | 89 ± 6 |

F95582 | 7.8 ± 5.4^{e} | 3.0 ± 2.6^{e} | 1.6 ± 1.6^{f} | 1.9 ± 1.1 | 85 ± 9 |

039nm | |||||

F75581 | 5.8 ± 3.0^{e} | 0.8 ± 0.3^{e} | 1.1 ± 0.3^{e} | 0.7 ± 0.1^{e} | 25 ± 8^{e} |

F51444 | 12.5 ± 7.0^{f} | 2.4 ± 1.1^{e} | 2.1 ± 0.6^{e} | 1.1 ± 0.2^{e} | 84 ± 16 |

JR-CSF | |||||

F104995 | 6.6 ± 4.0^{e} | 1.4 ± 1.2^{e} | 1.9 ± 0.8^{e} | 0.5 ± 0.2^{e} | 71 ± 16^{f} |

F104996 | 15.3 ± 5.0 | 3.0 ± 1.4^{e} | 6.1 ± 2.5 | 0.5 ± 0.2^{e} | 11 ± 3^{e} |

YU10x | |||||

F92751 | 11.3 ± 2.0^{e} | 5.3 ± 1.0^{e} | 3.1 ± 0.6^{f} | 1.7 ± 0.2^{e} | 97 ± 3 |

F92754 | 12.9 ± 5.5^{f} | 6.2 ± 1.8 | 2.8 ± 0.9^{f} | 2.1 ± 0.2 | 94 ± 6 |

SF-2 | |||||

F92748 | 5.9 ± 4.0^{e} | 0.3 ± 0.2^{e} | 0.9 ± 0.1^{e} | 0.3 ± 0.2^{e} | 44 ± 26^{e} |

F92749 | 20.3 ± 15.0 | 9.3 ± 6.0 | 4.1 ± 3.8 | 2.7 ± 0.6 | 99 ± 9 |

T71R | |||||

F101376 | 11.5 ± 3.0^{f} | 5.4 ± 1.0^{e} | 1.9 ± 0.5^{e} | 2.8 ± 0.4 | 98 ± 2 |

F101374 | 9.3 ± 2.0^{e} | 3.7 ± 1.0^{e} | 2.4 ± 0.4^{e} | 1.5 ± 0.3^{e} | 65 ± 20^{f} |

F101371 | 28.4 ± 5.5 | 14.1 ± 3.0 | 5.9 ± 1.0 | 3.0 ± 0.3 | 95 ± 7 |

F86707 | 8.8 ± 1.5^{e} | 1.3 ± 0.3^{e} | 2.2 ± 0.3^{e} | 1.0 ± 0.1^{e} | 21 ± 2^{e} |

Non-Tg^{d} | 19.5 ± 3.5 | 9.3 ± 2.5 | 4.3 ± 1.0 | 2.2 ± 0.4 | 100 |

NL4-3(WT) | 6.3 ± 3.6^{e} | 1.2 ± 0.5^{e} | 1.7 ± 0.9^{e} | 0.6 ± 0.1^{e} | 24 ± 11^{e} |

↵

*a*FACS analysis was performed on 4 to 10 mice (1.5 to 4 months old) for each Tg line, including the positive control CD4C/HIV-Nef^{NL4-3(WT)}Tg line. Data from Tg mice were always compared to those of their littermates done the same day.↵

*b*The absolute cell number was calculated by multiplying the total number of cells in each organ by the percentage of each cell subset in this organ.↵

*c*The mean fluorescences for CD4 were obtained by calculating the ratio of CD4 staining in Tg mouse thymuses relative to that of non-Tg mouse thymuses (100%) done in the same experiment, the same day. Mean values were then calculated with the values for each line.↵

*d*The non-Tg control values were obtained by pooling the results of all non-Tg littermates from different lines.↵

*e*Significant decrease (*P*< 0.01) according to Student's*t*test.↵

*f*Significant decrease (*P*< 0.05) according to Student's*t*test.