TABLE 3.

Passage details of CWD-positive cervid brain homogenates inoculated into various hamster and wild-type mouse species

CWD inoculumSpecies inoculatedaNo. of animals with TSE signs/no. inoculatedbNo. of blot-positive animals/no. testedIncubation time of animals with TSE signs (mean dpi ± SD)cLife span (dpi, mean ± SD)Life span range (dpi)
eSg ha0/120/2None positive628 ± 98480-751
mdSg ha0/120/2None positive591 ± 98469-693
wdSg ha0/120/3None positive510 ± 95430-644
eDjun ha0/4Not doneNone positive500 ± 95384-615
mdDjun ha0/10Not doneNone positive507 ± 97399-659
wdDjun ha0/1Not doneNone positive476476
eChin ha6/84/5e626 ± 80730 ± 220555-1,133
mdChin ha6/86/7e648 ± 58690 ± 166563-1,133
wdChin ha0/6Not doneNone positive722 ± 239448-1,133
eSib ha0/2Not doneNone positive532 ± 69483-581
mdSib ha1/31/3e464482 ± 23464-509
wdSib ha1/41/4e735638 ± 118499-739
eArm ha1/41/2e745669 ± 121490-745
mdArm ha0/150/1None positive625 ± 96348-704
wdArm ha0/80/8None positive587 ± 40532-649
edRML mo0/90/9None positive592 ± 80488-736
mddRML mo0/60/6None positive679 ± 93488-805
wddRML mo0/60/6None positive730 ± 101562-893
eC57BL10 mo0/150/2None positive724 ± 107588-837
mdC57BL10 mo0/160/2None positive657 ± 169368-837
wdC57BL10 mo0/140/2None positive772 ± 125368-837
  • a Abbreviations are the same as those in Table 1. Djun, Djungarian; Chin, Chinese; Sib, Siberian; Arm, Armenian; RML mo, Rocky Mountain Laboratory mouse.

  • b For each group, 12 to 16 animals were inoculated. Animals lost due to intercurrent deaths (as defined in Table 1) are not included in these data.

  • c None positive, none of the animals in the group were TSE positive by either neurological signs or immunoblot analysis for brain PrP-res during their life span.

  • d RML mice were inoculated with the individual CWD-positive cervid brain homogenates used for both the Sg hamsters in Table 1 and the Tg (haPrP) mice in Table 2.

  • e The blot-positive animals also showed neurological signs consistent with TSE disease, while the blot-negative animals did not.