TABLE 1.

Mapping the binding epitopes of anti-fAPN RG4 and anti-mAPN R3-242 MAbsa

APN proteinRG4 MAb bindingR3-242 MAb binding
IFAFCIFAFC
Mock transfected
fAPN++
mAPN++
m/fAPN251-967++++
m/fAPN251-582++++
m/fAPN582-967++
m/fAPN1-582++
  • a BHK cells transiently transfected with plasmids encoding fAPN, mAPN, or chimeric m/fAPN proteins were tested for reactivity with anti-fAPN RG4 or anti-mAPN R3-242 MAbs by IFA and flow cytometry (FC).