TABLE 2.

Blockade of FCoV and HCoV-229E infection with the anti-fAPN RG4 MAb in cells expressing fAPN or chimeric m/fAPN proteinsa

APN proteinHCoV-229E infectionFCoV infection
Control MAbRG4 MAbControl MAbRG4 MAb
fAPN++
m/fAPN251-967++
m/fAPN251-582+NANA
m/fAPN582-967NANAb
m/fAPN1-582+NANA
  • a Infection with FCoV or HCoV-229E was detected by immunofluorescence with antiviral antibodies 16 to 18 h after inoculation. +, antibody blockade of virus infection (<5% of cells infected); −, no antibody blockade of virus infection (≥90% of cells infected). NA, not applicable; the m/fAPN chimeric protein has no receptor activity for the virus.

  • b This antibody does not bind to this m/fAPN chimera.