TABLE 1.

Antibody and viral titers after RSV challenge in mice previously immunized with FIRSVa

Mouse groupPRNT60Antibody titer for indicated assayRSV titer (log PFU/g)
EIA-FEIA-G
C3aR+/+<10683 ± 33366 ± 263.2 ± 0.03
C3aR−/−<10626 ± 42366 ± 363.0 ± 0.01
C5+/+<10403 ± 19343 ± 382.2 ± 0.03
C5−/−<10402 ± 16350 ± 452.3 ± 0.03
  • a Antibody responses in mice were determined 7 days after RSV challenge and RSV pulmonary titers 4 days after challenge. PRNT60, RSV-specific neutralization as measured by 60% complement-enhanced plaque reduction; EIA-F, immunoassay determination of IgG antibodies against RSV F protein (change in absorbance); EIA-G, immunoassay determination of IgG antibodies against RSV G protein (change in absorbance). Results are means ± standard errors of the means.