TABLE 2.

AAV sequence relatedness and DNA copy number in pediatric tissues

Sample (age, yr)aAmino acid identityb (%)Nucleotide identityc (%)AAV copies/μgd
T17 (2.3)AAV2 (98.1)AAV2 (96.0)560
T32 (5.5)AAV2 (98.1)AAV2 (96.0)210
T40 (2.9)AAV2 (98.0)AAV2 (96.1)6,550
T41 (2.6)AAV2 (98.0)AAV2 (96.0)590
T70 (3.2)AAV2 (98.4)AAV2 (96.8)7,800
T71 (5.8)AAV2 (98.0)AAV2 (96.0)7,600
T88 (4.7)AAV2 (98.0)AAV2 (96.5)33,000
S17 (8)AAV3 (92.7)AAV2 (90.0)200
LG15 (1)AAV2 (98.4)AAV2 (96.8)80
  • a T = tonsil/adenoid; S = spleen; LG = lung. The age of the subject in years is in parentheses.

  • b Percent amino acid identity to the indicated AAV serotype is given in parentheses.

  • c Percent nucleotide identity to the indicated AAV serotype is given in parentheses.

  • d AAV DNA copy number determined using Q-PCR (see Materials and Methods). Values shown are the average of two separate determinations.