TABLE 3.

Heparin sulfate column binding of AAV2 virus preparations

VirusNonbound DRPaBound DRPbPercent Bound
AAV22.4 × 1091.5 × 10100.86
T70-432.0 × 10101.0 × 1090.05
T88-411.7 × 10101.4 × 1090.08
  • a DNase-resistant particle (DRP) copy number was determined using Taqman Q-PCR. Primers and probe were homologous to a conserved cap sequence (see Materials and Methods). Nonbound represent total virus found in wash and flowthrough material. Data shown are the average of two experiments.

  • b Copy number present in pooled 1-ml gradient fractions. Data shown are the average of two experiments.