TABLE 3.

Quantification of IFN-induced gene expression in HuH6 cells with and without HCV replicons

Genea and cell lineSignalbFold changec
MockIFN-αIFN-γIFN-αIFN-γ
HuH6 Con1 replicon cells
    GBP12.4189337.6395
    IP100.71910626147
    MxA1418338132.7
    OAS11.31,641151,29112
    STAT13212,2673,3127.110
HuH6 JFH-1 replicon cells
    GBP11.84022323126
    IP100.53303964877
    MxA183973712244.0
    OAS13.84553371,2119.7
    STAT12224,2781,366196.2
Naive HuH6 cells
    GBP10.83.75864.9768
    IP100.15.51761053,325
    MxA2.9563181966.3
    OAS11.01,943222,04123
    STAT13493,4673,5659.910
  • a Database accession numbers are given in Materials and Methods.

  • b mRNA copy number per 100,000 β-actin mRNA copies 24 h after IFN treatment.

  • c Signal in IFN-treated cells per signal in mock-treated cells.