TABLE 4.

Influence of inhibitors of N-glycosylation on the infectious yields of the generated recombinant viruses

HA variantaNAbExpt no.Infectious virus yield (TCID50/ml)
Control+ Swainsoninec+ Deoxymannojirimycind
G1,2,3NA15 × 1061 × 1072 × 106
27 × 1065 × 1062 × 106
G1,2,3delNA/eGFP12 × 1056 × 1054 × 102
22 × 1066 × 1057 × 102
G3delNA/eGFP14 × 1041 × 104<101
25 × 1052 × 1045 × 101
  • a The plasmid used encoded either the wild-type HA of the A/Charlottesville/31/95 virus (G1,2,3) or its variant (G3) lacking glycans at 94a and 129.

  • b The plasmid used in the rescue experiments encoded either the wild-type NA (A/WSN/33) (a control) or its defective variant (pdelNA/eGFP).

  • c Final concentration, 60 μM (10 μg/ml).

  • d Final concentration, 75 μM (15 μg/ml).