TABLE 1.

Infectivities of a wild-type construct (pS81-SA), an intermediate construct (pS81-SAS12), and a full HC-Pro deletion construct (pS81ΔHC12-5) of WSMV on three hostsa

ConstructNo. of plants infected/no. of plants inoculatedb
WheatOatSDp2 corn
TranscriptsProgeny virusProgeny virusProgeny virus
pS81-SA10/109/1010/106/610/109/108/108/9
pS81-SAS129/910/1016/1612/1210/1010/105/107/9
pS81ΔHC12-53/101/1016/1613/138/106/109/106/9
None0/100/100/160/100/100/100/100/9
  • a Infectivities and genotypes were verified by RT-PCR.

  • b Plants were mechanically inoculated with RNA derived from cloned WSMV genomes (transcripts) or with sap extracted from infected wheat plants (progeny virus) previously inoculated with transcripts.