TABLE 4.

Neutralization phenotypes of pseudoviruses containing cloned Env from acute or early HIV-1 infections as determined with serum from HIV-1-infected individuals, sCD4, and MAbs.

VirusaPanel designationaID50 in TZM-bl cellsb
Reciprocal serum dilutionμg/ml
DUMC-1DUMC-2DUMC-3LW.0013TH.10.03Pool APool BPool CPool DNormal poolsCD4IgG1b122G122F54E10TriMab
MN15,2608,0831,5415,97211,32514,09543,2646,25310,181<200.010.01>500.010.010.01
SF162.LS>43,74043,7351,1789,75641,0736,305>43,74025,8183,442250.050.010.60.10.30.03
IIIB2,861744691,873265343803547757<200.010.011.00.20.20.04
6101.10SVPB11,636<20<2027<20418716138<206.0>503.0>500.310.1
5768.4SVPB2488<20<20405353209354122<204.03.09.0126.10.9
3988.25SVPB31,72929971541334263134771044512.00.40.3>500.50.8
7165.18SVPB4107<20<20<202217911935695604.3>500.93.91.11.2
6535.3*SVPB5*409337<201188227347846561<200.81.42.01.90.21.2
QH0692.42*SVPB6*46240685453853742371151050.50.32.81.01.40.3
QH0515.1SVPB7874<20<20<20<20<204712540<208.02.60.1105.00.1
SC422661.8*SVPB8*5,148<205123511363248317167964.70.22.10.70.90.3
SS1196.1SVPB93,916254801771293462,4571,742306480.80.312.0240.30.5
BG1168.1SVPB10305<2021<20<205858179462610.0>50>500.81.83.5
PVO.4*SVPB11*2,497<2050<20395010553457576.7>501.2>506.54.2
TRO.11*SVPB12*4,571<20<20131<202982827501682811.5>500.4>500.30.8
AC10.0.29*SVPB13*89<20<2081319010423540298.51.9>501.30.30.9
RHPA4259.7*SVPB14*1,473<2030947887188455117<201.80.1>5012.06.90.1
THRO4156.18*SVPB15*3307137147666911436755<200.30.5>50>500.31.0
REJO4541.67*SVPB16*3,63429054541227258503608465<200.50.7>500.60.70.5
TRJO4551.58*SVPB17*570<20<20147229711938397<2020.2>50>50>504.5>25
WITO4160.33*SVPB18*4,410<20<207334280214376216<205.43.11.10.60.30.7
CAAN5342.A2*SVPB19*423<2021775114310521573<2016.0>50>503.62.717.9
GMTc9362122654110718936197213.61.00.9
  • a Clones selected as standard reference strains are marked with an asterisk.

  • b Values are the dilution or concentration at which RLU were reduced 50% compared to those in virus control wells. DUMC-1, DUMC-2, DUMC-3, LW.0013, and TH.10.03 are serum samples from individuals infected with clade B HIV-1.

  • c Geometric mean titer against the 19 pseudoviruses containing cloned primary isolate Env (excludes MN, SF162.LS, and IIIB). Serum/plasma titers of < 20 were assigned a value of 10 for calculations. The TriMab ID50 of > 25 against TRJO4551.58 was assigned a value of 50. Due to multiple ID50 values of > 50, the GMT was not determined for IgG1b12, 2G12, and 2F5.