TABLE 1.

Evaluation of T-20 and T-649 sensitivity of PM1-M87-selected isolates (NL/sel and BaL/sel) and pseudotyped variants carrying envelope proteins with point mutations (NL/S [L33S], BaL/V [I48V], BaL/K [N126K], BaL/VK [I48V/N126K]) in TZM-bl indicator cellsa

IsolateRelative fusion inhibitor resistance
T-20T-649
NL4-311
NL/sel8.0 ± 1.040.2 ± 6.3
NL/S10.1 ± 4.350.0 ± 5.7
BaL11
BaL/sel16.2 ± 3.35.3 ± 1.2
BaL/V2.4 ± 0.91.0 ± 0.4
BaL/K5.6 ± 1.35.3 ± 0.8
BaL/VK5.6 ± 1.52.5 ± 0.5
  • a The mean IC50 values were calculated from at least three independent TZM-bl infections. Relative fusion inhibitor resistance was calculated by dividing the IC50 value for the PM1-M87-selected isolates and each envelope protein variant by the IC50 value for HIV-1NL4-3 or HIV-1BaL.