TABLE 1.

Comparaison of the epitopes recognized by anti-Tat antibodies induced by CyaA-E5-Tat and Tat toxoid

Immunization protocolResponse to Tat peptides containing amino acidsa:
1-2021-4036-5046-6556-7065-8073-86
3× CyaA-E5-Tat (10μg, i.p.)3.0b3.8
3.03.2
4.24.4
4.03.9
4.05.4
3× CyaA-E5-Tat (10 μg, s.c.)5.63.8
2.6
5.5
4.2
4.2
3× Tat toxoid (10 μg, s.c.)4.62.7
4.22.9
4.2
4.53.43.0
5.1
3× Tat toxoid (10 μg) + alum (s.c.)4.1
5.23.13.0
4.64.2
4.44.22.5
4.13.1
3× CyaA-E5-Tat (10 μg, i.d.)4.92.8
4.14.0
3.62.6
5.43.3
5.63.4
3× Tat toxoid (10 μg, i.d.)4.6
3.9
4.2
4.94.1
4.14.12.8
3× CyaA-E5-Tat (10 μg, s.c.) (day, +180)4.73.1
5.23.9
4.83.6
3.83.5
3.73.6
3× Tat toxoid (10 μg) + alum (s.c.) (day, +180)4.33.72.5
3.83.82.6
4.45.02.5
5.33.63.0
2.32.5
  • a The sera of five mice per group were collected and tested for their response to the indicated Tat peptides. The results are expressed as individual Tat peptide-specific IgG titers (log).

  • b —, no Tat peptide-specific IgG antibodies were detectable.