TABLE 2.

Linear random-effects model to evaluate divergence of PBMC-associated HIV-1 sequence from the MRCA during HAARTa

SubjectSlope (P value)
pol PRpol RTenv C2-V5
F1−0.00012(0.8)0.00019(0.8)0.00128(0.5)
G20.00210(<0.00001)0.00229(0.00002)0.00683(<0.00001)
H2−0.00022(0.6)−0.00008(0.9)−0.00107(0.4)
H40.00008(0.9)0.00036(0.7)−0.00143(0.6)
L1−0.00048(0.3)−0.00039(0.5)−0.00191(0.2)
M20.00002(1.0)−0.00036(0.5)−0.00076(0.6)
P1−0.00023(0.7)−0.00026(0.8)0.00025(0.9)
R1−0.00034(0.4)0.00004(0.9)−0.00121(0.4)
S1−0.00014(0.9)−0.00051(0.6)−0.00135(0.6)
T20.00010(0.9)0.00004(1.0)No env sequences
  • a Positive slopes indicate an accumulation of mutations from viral replication, while negative slopes suggest a loss of recently evolved viral sequences from the infected PBMC. In this model, viral replication was detected in the PBMC of G2 only. Positive slopes in env suggest immune selection, while positive slopes in pol suggest antiretroviral selection.