TABLE 1.

Targeting efficiency for the second CBF1 allele in DG75

Expt% GFP+aTotal no. of clones grownbNo. of GFP+ clones testedCorrect integrationc
A3186835 (1)
B4145755 (1)
C423914210 (2)
  • a Percentage of EGFP-positive cells as determined by FACS analysis 2 days after transfection.

  • b Clones growing under selection on 96-well plates, counted 2.5 weeks later.

  • c Retargeting of the first allele is indicated by the number in parentheses.