Activation of the human IFN-β promoter and ISRE-dependent transcription, following infection with HSV-1a

  • a Cultures of neuro-2A cells were transfected with the designated promoter constructs (1 μg) plasmid DNA. At 24 h after transfection, the cultures were infected with the designated viruses using an MOI of 4. At 40 h after transfection, the total cell lysate was collected and CAT activity was measured. The levels of acetylated chloramphenicol was measured using a BioRad Molecular Imager FX. The levels of promoter activity in mock-infected cells were set and the fold of stimulation was then calculated for each promoter. The results of hree independent experiments are shown for the two promoters. Four experiments (1 to 4) are shown for each construct.