TABLE 2.

Detailed analysis of G → A mutations in the protease gene of 19 hypermutated sequences from the resting CD4+ T cells of eight patients on HAART

nt positiona424647484951607388100108114118122125126142143144145146151152154178183193202203217232233256257260270280281
G → A contextbGANGGGGGGGGGGGGGCNGGNGANGANGANGANGCNGGNGANGGNGANGGGGGGGGGGGNGANGGNGANGGNGANGGNGANGGNGANGGNGGNGANGGNGANGANGANGGNGGN
99.E15AAA
99.E19AAAA
99.E36AAAAAAA
113.G2AAAAAAAAAAAAAAAAAAA
113.I6AAAAAAAAAA
113.I16AAAAAA
113.I49AAAAAAA
113.I105AAAAAAAAAAAAAAAAAAA
134.D1AAAAAAA
135.F36AAAAA
135.F39AAAAA
136.G2AAAAAAAA
139.F1AAAAAA
139.H1AAAAAAAA
147.E11AAAAAAA
147.E22AAAAAAA
154.F7AAAAAAAAAAAA
154.F23AAAAAAAAAAAAAA
154.G17AAAAAAAAAAA
% Of sequences with mutation5161621115321111213255563*11374253261153547111116325422657416165685
Amino acid positionc4248528694
SubstitutiondW-*G-KG-SG-RG-S
  • a The nucleotide (nt) position of the hypermutated G within the protease gene is given. Nucleotide positions where more than 50% of the hypermutated sequences contained G → A mutations are indicated in boldface type. Designations in first column represent sequences.

  • b The hypermutated G (underlined) and following two 3′ nucleotides are shown.

  • c The corresponding amino acid position within the protease gene where hot spot G → A mutations occur is shown.

  • d The amino acid substitution occurring as a result of a hot spot G → A mutation. A stop codon is labeled with an asterisk (*).